You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-alpha 1 Antichymotrypsin antibody (ab9374)

Submit an Abreview Q&A (2)

Overview

  • Product nameAnti-alpha 1 Antichymotrypsin antibodySee all alpha 1 Antichymotrypsin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha 1 Antichymotrypsin
  • SpecificityThe antibody is specific against human alpha-1-antichymotrypsin. This antibody stains monocytes and macrophages in human tissues and does not stain any other cell type.
  • Tested applicationsIHC-Fr, IHC-P, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals

    Does not react with

    Mouse
  • Immunogen

    Alpha-1-antichymotrypsin isolated from human serum

Properties

Applications

Our Abpromise guarantee covers the use of ab9374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr IHC-Fr: 1/25 - 1/50. ABC method.
IHC-P IHC-P: 1/25 - 1/50. ABC method.
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) as Capture.
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa).

Target

  • FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01011 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        SERPINA3 antibodyAACT antibodyAACT_HUMAN antibody
        ACT antibodyAlpha 1 Antichymotrypsin 1 antibodyAlpha 1 Antichymotrypsin 2 antibodyAlpha 1 Antichymotrypsin 3 antibodyAlpha 1 Antitrypsin Deficiency antibodyAlpha-1-antichymotrypsin His-Pro-less antibodyAnti Elastase antibodyAntichymotrypsin antibodyCell growth inhibiting gene 24/25 protein antibodyCell growth-inhibiting gene 24/25 protein antibodyClade A (Alpha 1 Antiproteinase Antitrypsin) antibodyGIG24 antibodyGIG25 antibodyGrowth inhibiting protein 24 antibodyGrowth inhibiting protein 25 antibodyMGC88254 antibodyPI1 antibodyProtease Inhibitor 1 antibodySerine (or Cysteine) Proteinase Inhibitor antibodySerine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibodySerine (or cysteine) proteinase inhibitor clade A member 3 antibodySerine proteinase inhibitor clade A member 3 antibodySerpin A3 antibodySerpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibodySERPINA1 antibodySERPINA2 antibodySERPINA3 antibody
      see all

    Anti-alpha 1 Antichymotrypsin antibody images

    • Sandwich ELISA - alpha 1 Antichymotrypsin antibody (ab9374)
      Sandwich ELISA - alpha 1 Antichymotrypsin antibody (ab9374)
      Standard Curve for alpha 1 Antichymotrypsin (Analyte: alpha 1 Antichymotrypsin protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) at 0.5µg/ml.
    • Western blot - alpha 1 Antichymotrypsin antibody (ab9374)
      Western blot - alpha 1 Antichymotrypsin antibody (ab9374)
      All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab9374) at 1 µg/ml

      Lane 1 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
      Lane 2 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 48 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 1 Antichymotrypsin antibody (ab9374)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 1 Antichymotrypsin antibody (ab9374)
      Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel: with primary antibody at 2 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Western blot
      Western blot
      Anti-alpha 1 Antichymotrypsin antibody (ab9374) at 1 µg/ml + alpha 1 Antichymotrypsin protein (Human) (ab80517) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    Protocols

    References for Anti-alpha 1 Antichymotrypsin antibody (ab9374)

    ab9374 has not yet been referenced specifically in any publications.

    Publishing research using ab9374 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab9374

    Displaying 1 - 2 of 2 results for Q&A

    10
    Votes: Highest First

    What is the buffer solution for this antibody? The spec sheet states that the antibody has been protein A purified, however, the buffer solution is antiserum as opposed to PBS or some other buffer. Could you clear that up? Also, does the sample buffer ...

    Read More

    Thank you for your enquiry. The antibody is suspended in PDB and it contains BSA. PDB buffer is PBS supplemented with 1.5 mM MgCl2, 0.1 mM dithiothreitol, 0.01% bovine serum albumin. I have updated this on the datasheet for future reference. Pleas...

    Read More

    What is your recommended ELISA concentration for this antibody? If possible, I need this information in time for a meeting in one hour. Thank you!

    Thank you for your email. This antibody has not yet been tested in ELISA. So far, it has only been characterized in Immunohistochemistry - both formalin-fixed paraffin-embedded and frozen sections. If you have any more questions, please contact us again...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"