Anti-NUSAP1 antibody (ab93779)

Overview

• Product nameAnti-NUSAP1 antibodySee all NUSAP1 primary antibodies ...
• Description
  Rabbit polyclonal to NUSAP1
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide from a region within amino acids 125 - 175 of Human NUSAP1 (NP_057443.2)

• Positive controlHeLa cell lysate 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: Tris citrate/phosphate, pH 7 to 8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Cytokinesis

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

Applications

Target

• FunctionMicrotubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
• Sequence similaritiesBelongs to the NUSAP family.
• DomainThe KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
  Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
• Cellular localizationCytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.

Target information above from: UniProt accession Q9BXS6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51203 Human
  • Entrez Gene: 51203 Human
  • Omim: 612818 Human
  • SwissProt: Q9BXS6 Human
  • SwissProt: Q9BXS6 Human
  • Unigene: 615092 Human

• Alternative names
  2610201A12Rik antibodyAI481307 antibodyANKT antibody

  AW547774 antibodyBB165529 antibodyBM037 antibodyFLJ13421 antibodyLNP antibodyNucleolar and spindle associated protein 1 antibodyNucleolar and spindle-associated protein 1 antibodyNucleolar protein ANKT antibodyNUSAP antibodyNUSAP_HUMAN antibodynusap1 antibodyPRO0310p1 antibodyQ0310 antibodyRP23-22A15.4 antibodySAPL antibody

  see all

Anti-NUSAP1 antibody images

• 

  Western blot - NUSAP1 antibody (ab93779)
  All lanes : Anti-NUSAP1 antibody (ab93779) at 0.4 µg/ml
  
  Lane 1 : HeLa cell lysate at 50 µg
  Lane 2 : HeLa cell lysate at 15 µg
  Lane 3 : HeLa cell lysate at 5 µg
  Lane 4 : 293T cell lysate at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 49 kDa
  
  
  Exposure time : 3 minutes
• 

  Immunoprecipitation - NUSAP1 antibody (ab93779)
  Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
  All lanes : NUSAP1 antibody (ab93779) at 1 µg/ml
  Lane 1 : Lysate immunoprecipitated with ab93779
  Lane 2 : Lysate immunoprecipitated with a control IgG
  
  Developed using the ECL technique
  Exposure time : 30 seconds