Anti-Interferon alpha 2b antibody [4E10] (ab9388)
Key features and details
- Mouse monoclonal [4E10] to Interferon alpha 2b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Interferon alpha 2b antibody [4E10]
See all Interferon alpha 2b primary antibodies -
Description
Mouse monoclonal [4E10] to Interferon alpha 2b -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Interferon alpha 2b.
Database link: P01563 -
General notes
The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR317571-2, GR317571-3 and GR190761-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4E10 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500.
|
Notes |
---|
WB
1/500. |
Target
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Function
Produced by macrophages, IFN-alpha have antiviral activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
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Alternative names
- Alpha 2a interferon antibody
- IFN alpha 2b antibody
- IFN-alpha-2 antibody
see all
Images
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Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4
µ g/ml).
Lane 1 - interferon alpha 2b
Lane 2 - prestained standard proteins.Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4
µg/ml).
Lane 1 - interferon alpha 2b
Lane 2 - prestained standard proteins.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab9388 has not yet been referenced specifically in any publications.