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Overview

  • Product nameNAP1L4 protein (Tagged-His Tag)See all NAP1L4 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 24-375 (352 amino acids) of Human NAP1L4 with an N terminal His tag. Predicted mwt: 42 kDa; UniProt ID = Q99733.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 95 µl aqua dest.
  • Storage instructionsStore at 4°C. Upon reconstitution store at -80ºC.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesTEKLTDQVMQNPRVLAALQERLDNVPHTPSSYIETLPKAV KRRINALKQLQVRCAHIEAKFY EEVHDLERKYAALYQP LFDKRREFITGDVEPTDAESEWHSENEEEEKLAGDMKSKV VVTEKA AATAEEPDPKGIPEFWFTIFRNVDMLSELVQE YDEPILKHLQDIKVKFSDPGQPMSFVLEFH FEPNDYFT NSVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKG KNVTVKTIKKKQKH KGRGTVRTITKQVPNESFFNFFNP LKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVL YFTGEAIEDDDNFEEGEEGEEEELEGDEEGEDEDDAEINP KV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab93980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        NAP2Nucleosome assembly protein 1-like 4Nucleosome assembly protein 2
  • RelevanceThis gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.
  • Cellular localizationNuclear

    • NAP1L4 protein (Tagged-His Tag) images

    • SDS-PAGE - NAP1L4 protein (Tagged-His Tag) (ab93980)
      SDS-PAGE - NAP1L4 protein (Tagged-His Tag) (ab93980)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for NAP1L4 protein (Tagged-His Tag) (ab93980)

    ab93980 has not yet been referenced specifically in any publications.

    Publishing research using ab93980 ? Please let us know so that we can cite the reference in this datasheet

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