Overview
- Product nameBonzo 293T Transfected Lysate - (positive control)
- General notesab94068 is a 293T cell transfected lysate in which Human Bonzo has been transiently over-expressed using a pCMV-Bonzo plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
- Tested applicationsWB more details
Properties
- FormLiquid
- Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
- Storage bufferConstituent: 100% 1x Sample Buffer
-
Concentration information loading... - PurityWhole Cell Lysate
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab94068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Bonzo 293T Transfected Lysate - (positive control) images
-
ab94068 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-Bonzo antibody (ab89579) at 1/500 dilution
Lane 1 :Bonzo 293T Transfected Lysate - (positive control) (ab94068) at 25 µg
Lane 2 : 293T non-transfected lysate
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Protocols
References for Bonzo 293T Transfected Lysate - (positive control) (ab94068)
ab94068 has not yet been referenced specifically in any publications.

