PAK1 protein (Active) (ab95253)
Different concentration of PAK1 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR14) at room temperature for 1 hour.
Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
Fluorescence was then detected using Lambda exc=460±40 nm and Lambda em=528±20 nm filters.
Constituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0
Our Abpromise guarantee covers the use of ab95253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- Alpha PAKAlpha-PAKMGC130000
- MGC130001p21 activated kinase 1p21 protein (Cdc42/Rac) activated kinase 1p21-activated kinase 1p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related)p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)p65 PAKp65-PAKPAK alphaPAK-1Pak1PAK1_HUMANPakaPAKalphaProtein kinase MUK2Rac/p21-activated kinaseSerine/threonine-protein kinase PAK 1STE20 homolog yeast
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
PAK1 protein (Active) images
Image showing specific activity of ab95253.
SDS-PAGE analysis of PAK1 (ab95253) and BSA at conc. of 8, 40, 80 and 160 ug/ml.
References for PAK1 protein (Active) (ab95253)
ab95253 has not yet been referenced specifically in any publications.