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Overview

  • Product namePAK1 protein (Active)See all PAK1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 248-545 of Human PAK1; 293 amino acids, 32.8kDa (predicted from sequence). UniProt ID = Q13153.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activitySpecific Activity: 32638 pmoles/min/µg. Analysis of enzymatic activity was performed according to Zlyte assay protocol:
    Different concentration of PAK1 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR14) at room temperature for 1 hour.
    Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
    Fluorescence was then detected using Lambda exc=460±40 nm and Lambda em=528±20 nm filters.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab95253 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Alpha PAKAlpha-PAKMGC130000
        MGC130001p21 activated kinase 1p21 protein (Cdc42/Rac) activated kinase 1p21-activated kinase 1p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related)p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)p65 PAKp65-PAKPAK alphaPAK-1Pak1PAK1_HUMANPakaPAKalphaProtein kinase MUK2Rac/p21-activated kinaseSerine/threonine-protein kinase PAK 1STE20 homolog yeast
      see all
  • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.

    • Target information above from: UniProt accession Q13153 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PAK1 protein (Active) images

    • Functional Studies - PAK1 protein (Active) (ab95253)
      Functional Studies - PAK1 protein (Active) (ab95253)
      Image showing specific activity of ab95253.
    • SDS-PAGE - PAK1 protein (Active) (ab95253)
      SDS-PAGE - PAK1 protein (Active) (ab95253)

      SDS-PAGE analysis of PAK1 (ab95253) and BSA at conc. of 8, 40, 80 and 160 ug/ml.

    References for PAK1 protein (Active) (ab95253)

    ab95253 has not yet been referenced specifically in any publications.

    Publishing research using ab95253 ? Please let us know so that we can cite the reference in this datasheet

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