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Overview

  • Product nameAPM2 peptide
  • Protein descriptionSynthetic peptide derived from C terminal region of human APM2 protein This peptide was used as an immunogen for ab79579 - APM2 antibody.

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • Additional notesIncubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 deg C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab95393 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution. This peptide has been used succesfully to create a standard curve in an ELISA using antibody APM2 antibody ab79579.
  • Application notesThis peptide can be used with studies using ab79579.

    • Protein info

    • Alternative names
        Adipose most abundant gene transcript 2 proteinAdipose specific 2APM 2
        C10orf116Chromosome 10 open reading frame 116OTTHUMP00000020019RP11-96C23.4
      see all
  • RelevanceAPM2 gene is exclusively expressed in adipose tissue. Its function is currently unknown. APM2 has been shown to be associated with cisplatin resistance in cell lines. It is not found in cisplatin sensitive cell lines, implicating APM2 in the development of cisplatin resistance in tumor cells.

    • APM2 peptide images

    • Indirect ELISA - APM2 peptide (ab95393)
      Indirect ELISA - APM2 peptide (ab95393)This image is courtesy of an anonymous Abreview
      ab95393 (serially diluted from 800 ng/ml and incubated for 1 hour at 24°C) was used for establishing the standard curve.

      ab79579 was used to detect APM2 in Human serum by indirect ELISA. Samples were blocked with 5% BSA for 1 hour at 24°C and were incubated with the primary antibody (1/1000) for 3 hours at 26°C. An HRP-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    References for APM2 peptide (ab95393)

    ab95393 has not yet been referenced specifically in any publications.

    Publishing research using ab95393 ? Please let us know so that we can cite the reference in this datasheet

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    ELISA Abreview for APM2 peptide

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    Abreviews
    Application ELISA

    Review text: 1. ab79579, APM2 antibody was used.
    2. APM2 peptide (ab95393) was serially diluted from 800 ng/ml .
    Sample: Human Serum (Serum)
    Specification: Serum
    Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Type: Indirect

    Other product details
    Concentration: 4 (unknown units)
    Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 24°C

    Primary antibody (in addition to 'APM2 peptide')
    Primary antibody: Abcam primary antibody: Anti-APM2 antibody (ab79579)
    Dilution: 1/1000

    Secondary antibody
    Name: Non-Abcam antibody was used: Anti-Rabbit IgG HRP Conjugated
    Host species: Donkey
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/1000
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    Verified customer

    Submitted Sep 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"