Anti-Aquaporin 1 antibody [1/22] (ab9566)

Overview

• Product nameAnti-Aquaporin 1 antibody [1/22]See all Aquaporin 1 primary antibodies ...
• Description
  Mouse monoclonal [1/22] to Aquaporin 1
• SpecificityThis antibody recognises Aquaporin 1
• Tested applicationsWB, ELISA, IHC-P, IHC-Fr, ICC, Flow Cyt, IHC-FoFr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Sheep, Cow
• Immunogen

  Synthetic peptide: GQVEEYDLDDDINSRVEMKPK, corresponding to amino acids 249-269 of Aquaporin 1.

  Run BLAST with Run BLAST with
• EpitopeIntracellular C-terminal AQP 1 epitope (active channel loop)

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: PBS, 2mg/ml BSA
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number1/22
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

Applications

Target

• FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
• Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
• Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
• DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
• Cellular localizationMembrane.

Target information above from: UniProt accession P29972 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282653 Cow
  • Entrez Gene: 358 Human
  • Entrez Gene: 11826 Mouse
  • Entrez Gene: 25240 Rat
  • Entrez Gene: 442999 Sheep
  • Omim: 107776 Human
  • SwissProt: P47865 Cow
  • SwissProt: P29972 Human

  • SwissProt: Q02013 Mouse
  • SwissProt: P29975 Rat
  • SwissProt: P56401 Sheep
  • Unigene: 76152 Human
  • Unigene: 18625 Mouse
  • Unigene: 1618 Rat

  see all

• Alternative names
  AQP 1 antibodyAQP CHIP antibodyAQP-1 antibody

  AQP1 antibodyAQP1_HUMAN antibodyAquaporin CHIP antibodyAquaporin-1 antibodyAquaporin-CHIP antibodyAquaporin1 antibodyChannel forming integral protein 28kDa antibodyChannel like integral membrane protein 28 kDa antibodyCHIP 28 antibodyCHIP28 antibodyCO antibodyColton blood group antibodyGrowth factor induced delayed early response protein antibodyMGC26324 antibodyUrine water channel antibodyWater channel protein CHIP 29 antibodyWater channel protein CHIP29 antibodyWater channel protein for red blood cells and kidney proximal tubule antibody

  see all

Anti-Aquaporin 1 antibody [1/22] images

• 

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Aquaporin 1 antibody [1/22] (ab9566)
  Immunohistochemical staining of aquaporin 1 in rat brain.
• 

  Western blot - Aquaporin 1 antibody [1/22] (ab9566)
  Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 29 kDa
  Observed band size : 29 kDa
  Additional bands at : 50 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds
• 

  Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)
  Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.