Anti-Ephrin B2 antibody (ab96264)

Overview

• Product nameAnti-Ephrin B2 antibodySee all Ephrin B2 primary antibodies ...
• Description
  Rabbit polyclonal to Ephrin B2
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Ephrin B2.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in Human lung and Human kidney tissue lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Angiogenic growth factors

  • Cardiovascular
  • Angiogenesis
  • Ephrins
  • Ephrins

  • Neuroscience
  • Neurotransmission
  • Intracellular Signaling
  • Kinases

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • Tyrosine Kinase Receptors

  • Neuroscience
  • Neurology process
  • Growth and Development
  • Axonal Guidance Proteins

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Growth Cone

Applications

Target

• FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
• Tissue specificityLung and kidney.
• Sequence similaritiesBelongs to the ephrin family.
  Contains 1 ephrin RBD (ephrin receptor-binding) domain.
• Post-translational
  modificationsInducible phosphorylation of tyrosine residues in the cytoplasmic domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession P52799 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1948 Human
  • Entrez Gene: 306636 Rat
  • Omim: 600527 Human
  • SwissProt: P52799 Human
  • Unigene: 149239 Human
  • Unigene: 219981 Rat

• Alternative names
  EFN B2 antibodyEFNB 2 antibodyEFNB2 antibody

  EFNB2_HUMAN antibodyEph related receptor tyrosine kinase ligand 5 antibodyEPH-related receptor tyrosine kinase ligand 5 antibodyephrin B2 antibodyEphrin-B2 antibodyEphrinB2 antibodyEPLG 5 antibodyEPLG5 antibodyHtk L antibodyHTK ligand antibodyHTK-L antibodyHTKL antibodyLERK 5 antibodyLERK-5 antibodyLERK5 antibodyLigand of eph related kinase 5 antibodyMGC126226 antibodyMGC126227 antibodyMGC126228 antibodyOTTMUSP00000024973 antibody

  see all

Anti-Ephrin B2 antibody images

• 

  Western blot - Ephrin B2 antibody (ab96264)
  All lanes : Anti-Ephrin B2 antibody (ab96264) at 1 µg/ml
  
  Lane 1 : Lung (Human) Tissue Lysate
  Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 36 kDa
  Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 73 kDa,86 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 12 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Ephrin B2 antibody (ab96264)
  ICC/IF image of ab96264 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96264, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.