Anti-GM-CSF antibody (ab9667)
Key features and details
- Rabbit polyclonal to GM-CSF
- Suitable for: Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-GM-CSF antibody
See all GM-CSF primary antibodies -
Description
Rabbit polyclonal to GM-CSF -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human GM-CSF. Highly pure (>98%) recombinant hGM-CSF (human Granulocyte Macrophage Colony Stimulating Factor)
Database link: P04141 -
Positive control
- Recombinant human GM-CSF protein (ab9668) can be used as a positive control in WB.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. -
Storage buffer
No preservative, sterile filtered -
Carrier free
Yes -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
Allows the detection of 0.2 - 0.4 ng/well of recombinant hGM-CSF (using 100µl/well antibody solution). Can be paired for Sandwich ELISA with ab84259. Can be used as the capture antibody when paired with recommended pair. |
Notes |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hGM-CSF (using 100µl/well antibody solution). Can be paired for Sandwich ELISA with ab84259. Can be used as the capture antibody when paired with recommended pair. |
Target
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Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
Sequence similarities
Belongs to the GM-CSF family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Colony stimulating factor 2 (granulocyte-macrophage) antibody
- Colony Stimulating Factor 2 antibody
- Colony stimulating factor antibody
see all
Images
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Sandwich ELISA of ab9667. Allows the detection of 0.2 - 0.4 ng/well of recombinant hGM-CSF (using 100µl/well antibody solution). Can be paired for Sandwich ELISA with ab84259. Can be used as the capture antibody when paired with recommended pair.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab9667 has been referenced in 2 publications.
- Sielska M et al. Tumour-derived CSF2/granulocyte macrophage colony stimulating factor controls myeloid cell accumulation and progression of gliomas. Br J Cancer N/A:N/A (2020). PubMed: 32390004
- Uchida K et al. Granulocyte/macrophage colony-stimulating factor autoantibodies and myeloid cell immune functions in healthy individuals. Blood : (2008). PubMed: 18845791