Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)

Overview

• Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbedSee all IgG secondary antibodies ...
• Target speciesRabbit
• SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
• Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
• Minimal
  cross-reactivity
  Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat
  To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
  more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Rabbit
  • IgG
  • Fluorophore
  • DyLight® 488

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed images

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  Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)
  ICC/IF image of (ab3280) stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3280, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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  Flow Cytometry - Anti-JAK3 antibody [EP909Y] (ab45141)
  Overlay histogram showing Jurkat cells stained with ab45141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45141, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  
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  DyLight® - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96899)
  Emission spectra of DyLight® fluorochromes available in our catalog.
  Line colors represent the approximate visible colors of the wavelength maxima.