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Anti-G-CSF antibody (ab9691)

Abreviews (1)Q&A (8)

Overview

  • Product nameAnti-G-CSF antibodySee all G-CSF primary antibodies ...
  • Description
    Rabbit polyclonal to G-CSF
  • Tested applicationsWB, ELISA, Neutralising, Sandwich ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hG-CSF (human G-CSF).

Properties

Applications

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA ELISA: Use at an assay dependent dilution. To detect hG-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hG-CSF.
Neutralising Neut: Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab9818 as Capture.
IHC-P IHC-P: Use a concentration of 0.25 µg/ml.

Target

  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P09919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Colony stimulating factor 3 (granulocyte) antibodyCSF 3 antibodyCSF beta antibody
        CSF3 antibodyCSF3_HUMAN antibodyCsfg antibodyFilgrastim antibodyG-CSF antibodyGCSA antibodyGCSF antibodyGranulocyte colony stimulating factor antibodyGranulocyte colony-stimulating factor antibodyLenograstim antibodyMacrophage granulocyte inducer 2 antibodyMGC45931 antibodyMGI 2 antibodyPluripoietin antibody
      see all

    Anti-G-CSF antibody images

    • Sandwich ELISA - G-CSF antibody (ab9691)
      Sandwich ELISA - G-CSF antibody (ab9691)
      Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - G-CSF antibody (ab9691)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - G-CSF antibody (ab9691)
      ab9691 staining G-CSF in human angiosarcoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
    • Western blot
      Western blot
      Anti-G-CSF antibody (ab9691) at 1 µg/ml + G-CSF protein (Active) (ab73371) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 2 minutes

    References for Anti-G-CSF antibody (ab9691)

    ab9691 has not yet been referenced specifically in any publications.

    Publishing research using ab9691 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-G-CSF antibody

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    Abreviews
    Application Western blot
    Sample Human Purified protein (E.coli)
    Loading amount 3 µg
    Specification E.coli
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Feb 11 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"