Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974)
Overview
- Product nameGoat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRat
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
- Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
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Minimal
cross-reactivity
Human, Mouse more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
- Clonality Polyclonal
- IsotypeIgG
- General notesDyLight® 650 replaces DyLight® 649.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab96974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/50 - 1/500. |
| ICC/IF | ICC/IF: 1/50 - 1/500. |
| Flow Cyt | Flow Cyt: 1/50 - 1/200. |
Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974)Abreview courtesy of Carl Hobbs, Kings's College LondonAnti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974) was used as a secondary antibody at a concentration of 1/500. -
DyLight®-Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed(ab96974)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References for Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974)
ab96974 has not yet been referenced specifically in any publications.
