You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.
AbExcel

Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)

Submit an Abreview Q&A (1)

Overview

  • Product nameRabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP)See all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WB more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notesMolar enzyme: antibody protein ratio is 1:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/100 - 1/1000.
ELISA ELISA: 1/5000 - 1/50000. (Primary).
WB WB: 1/5000 - 1/50000. (Chemiluminescent).

Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) images

  • Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)
    Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)
    All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane. 

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/20000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)

ab97097 has not yet been referenced specifically in any publications.

Publishing research using ab97097 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit a Question for ab97097

Displaying 1 - 1 of 1 results for Q&A

10
Votes: Highest First

To technical support,



I would like to order a complete set of antibodies and controls from Abcam for WB analysis. Could you check if these antibodies would suit for the analysis. In addition, could you recommend some positive contro...

Read More

Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102....

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"