Anti-IL-1 beta antibody (ab9722)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IL-1 beta antibody
See all IL-1 beta primary antibodies -
Description
Rabbit polyclonal to IL-1 beta -
Host species
Rabbit -
Specificity
Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.
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Tested applications
Suitable for: IHC-P, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
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Positive control
- IHC-P: Human adrenal tissue; Recombinant mouse IL-1 beta protein (ab9723) can be used as a positive control in WB
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (5) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. |
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WB | (6) |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Notes |
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. |
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
Tissue specificity
Expressed in activated monocytes/macrophages (at protein level). -
Sequence similarities
Belongs to the IL-1 family. -
Post-translational
modificationsActivation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
Cellular localization
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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Database links
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
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Alternative names
- Catabolin antibody
- H1 antibody
- IFN beta inducing factor antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 µg/ml.
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To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.
Non-reducing conditions.
Protocols
Datasheets and documents
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Datasheet download
References (764)
ab9722 has been referenced in 764 publications.
- Zheng G et al. The Selective NLRP3-inflammasome inhibitor MCC950 Mitigates Post-resuscitation Myocardial Dysfunction and Improves Survival in a Rat Model of Cardiac Arrest and Resuscitation. Cardiovasc Drugs Ther 37:423-433 (2023). PubMed: 34973094
- Ye T et al. Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity. Bioact Mater 21:194-208 (2023). PubMed: 36101856
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Li X et al. Ginsenoside Rg1 alleviates learning and memory impairments and Aβ disposition through inhibiting NLRP1 inflammasome and autophagy dysfunction in APP/PS1 mice. Mol Med Rep 27:N/A (2023). PubMed: 36367174
- Satitsri S et al. Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model. BMC Vet Res 19:4 (2023). PubMed: 36624444