Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
- Product nameAnti-SCP3 antibody [Cor 10G11/7]See all SCP3 primary antibodies ...
- DescriptionMouse monoclonal [Cor 10G11/7] to SCP3
- SpecificityThis antibody is an excellent tool for the visualisation of chromosomes in meiotic spreads.
- Tested applicationsIHC-P, ELISA, ICC/IF, IHC-Fr, WB more details
- Species reactivityReacts with: Mouse, Hamster, Zebrafish
Hamster COR1/SCP3 protein (expressed as 6x His fusion in bacteria and purified on a Talon under denaturing conditions).
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Monoclonal
- Clone numberCor 10G11/7
- Research Areas
Our Abpromise guarantee covers the use of ab97672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use at an assay dependent concentration.|
|ELISA||ELISA: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent dilution. Predicted molecular weight: 28 kDa.|
- FunctionComponent of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
- Tissue specificityTestis-specific.
- Involvement in diseaseDefects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
- Sequence similaritiesBelongs to the XLR/SYCP3 family.
- Cellular localizationNucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
- choline phosphotransferase 1 antibodychpt1 antibodyCOR 1 antibody
- COR1 antibodyMGC71888 antibodyRNASCP3 antibodySCP 3 antibodySCP-3 antibodySycp 3 antibodySycp3 antibodySYCP3_HUMAN antibodySynaptonemal complex protein 3 antibody
Anti-SCP3 antibody [Cor 10G11/7] images
ab97672 staining SCP3 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
ab97672 staining SCP3 in mouse embryonic day 16.5 female germ cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in Formaldehyde/SDS and permeabilized in 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS prior to blocking in 10% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-Mouse IgG polyclonal, diluted 1/400.
IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.
References for Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
This product has been referenced in:
- Heaney JD et al. Germ cell pluripotency, premature differentiation and susceptibility to testicular teratomas in mice. Development 139:1577-86 (2012). ICC/IF ; Mouse . Read more (PubMed: 22438569) »
- Barry KA et al. Geminin is required for mitotic proliferation of spermatogonia. Dev Biol 371:35-46 (2012). Read more (PubMed: 22898305) »