Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

Overview

• Product nameAnti-SCP3 antibody [Cor 10G11/7]See all SCP3 primary antibodies ...
• Description
  Mouse monoclonal [Cor 10G11/7] to SCP3
• SpecificityThis antibody is an excellent tool for the visualisation of chromosomes in meiotic spreads.
• Tested applicationsIHC-P, ELISA, ICC/IF, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Mouse, Hamster, Zebrafish
  
• Immunogen

  Hamster COR1/SCP3 protein (expressed as 6x His fusion in bacteria and purified on a Talon under denaturing conditions).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone numberCor 10G11/7
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Chromatid Cohesion

Applications

Target

• FunctionComponent of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
• Tissue specificityTestis-specific.
• Involvement in diseaseDefects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
• Sequence similaritiesBelongs to the XLR/SYCP3 family.
• Cellular localizationNucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.

Target information above from: UniProt accession Q8IZU3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 20962 Mouse
  • Entrez Gene: 322605 Zebrafish
  • SwissProt: P70281 Mouse
  • Unigene: 297977 Mouse

• Alternative names
  choline phosphotransferase 1 antibodychpt1 antibodyCOR 1 antibody

  COR1 antibodyMGC71888 antibodyRNASCP3 antibodySCP 3 antibodySCP-3 antibodySycp 3 antibodySycp3 antibodySYCP3_HUMAN antibodySynaptonemal complex protein 3 antibody

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Anti-SCP3 antibody [Cor 10G11/7] images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Qin Wen

  ab97672 staining SCP3 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Jason Heaney.
  ab97672 staining SCP3 in mouse embryonic day 16.5 female germ cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in Formaldehyde/SDS and permeabilized in 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS prior to blocking in 10% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-Mouse IgG polyclonal, diluted 1/400.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an abreview by Zachary Yu-Ching Lin.
  IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.

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