Overview
Product nameBIN1 proteinSee all BIN1 proteins and peptides ...
Protein descriptionRecombinant full length Human BIN1 (amino acids 1-439) with an N terminal His tag; 459 amino acids including the tag, MWt 50.4kDa. (UniProt O00499-7, Isoform II3).
Uniprot accessionO00499
Molecular weight50.400kDa inclusive of tags
Protein length439 amino acids
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesab98238 was purified using conventional chromatography techniques.
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0
Concentration information loading...
SequenceMGSSHHHHHHSSGLVPRGSHMAEMGSKGVTAGKIASNVQK KLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTR LQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEA NKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRI AKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKAEEELIK AQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEEN FHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPSDNAPA KGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKS PSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVV VETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTAT DTDELQLRAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQH KELEKCRGVFPENFTERVP
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab98238
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent dilution. |
Protein info
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Alternative names
Amphiphysin IIAmphiphysin like proteinamphiphysin-likeAmphiphysin-like proteinAMPHLBin1BIN1_HUMANBox Dependant MYC Interacting Protein 1Box-dependent myc-interacting protein 1Bridging Integrator 1DKFZp547F068MGC10367MGC105358Myc box dependent interacting protein 1Myc box-dependent-interacting protein 1SH3P9
see all
FunctionMay be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Tissue specificityUbiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Involvement in diseaseDefects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
Post-translational
modificationsPhosphorylated by protein kinase C.
Cellular localizationCytoplasm and Nucleus.
References for BIN1 protein (ab98238)
ab98238
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - BIN1 protein (ab98238)15% SDS-PAGE analysis of 3µg ab98238