Overview
Product nameCardiotrophin 1 protein (Active)See all Cardiotrophin 1 proteins and peptides ...
Protein descriptionHighly pure (>98%) recombinant human Cardiotrophin 1.
Expression hostE. coli
Properties
Purification notesSterile filtered
Greater than 99% pure by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
FormLyophilised:The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. We recommend a quick spin followed by reconstitution in 20 mM Tris, pH 8.0 to a concentration of < 1.0mg/ml.
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Concentration information loading...
Additional notesThe ED50, as determined by the dose-dependent proliferation of TF-1 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg. The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. We recommend a quick spin followed by reconstitution in 20 mM Tris, pH 8.0 to a concentration of < 1.0mg/ml.
Sequence notesRecombinant Human Cardiotrophin-1 is a 21.5 kDa protein containing 201 amino acid residues:
MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQ EYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCR RQAE LNPRAPRLLRRLEDAARQAR ALGAAVEALL AAL GAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab9838
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| Functional Studies |
FuncS: Use at an assay dependent dilution. |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
-
Alternative names
Cardiophin 1Cardiophin1Cardiotrophin 1
Cardiotrophin ICardiotrophin-1Cardiotrophin1CardiotrophinICT 1CT-1CT1CTF 1CTF1CTF1_HUMAN
see all
FunctionInduces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Tissue specificityHighly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
Sequence similaritiesBelongs to the IL-6 superfamily.
Cellular localizationSecreted.
References for Cardiotrophin 1 protein (Active) (ab9838)
ab9838
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
