Goat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (AP) (ab98522)
Overview
- Product nameGoat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (AP)See all IgG secondary antibodies ...
- Target speciesHuman
- Tested applicationsICC, IHC-P, ELISA, WB more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab98522 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline Phosphatase.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab98522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent dilution. |
| IHC-P | IHC-P: 1/100 - 1/1000. |
| ELISA | ELISA: 1/1000 - 1/10000. as primary |
| WB | WB: 1/1000 - 1/10000. by chemiluminescent detection |
References for Goat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (AP) (ab98522)
ab98522 has not yet been referenced specifically in any publications.
