Anti-Tbx3 antibody (ab99302)

Overview

• Product nameAnti-Tbx3 antibodySee all Tbx3 primary antibodies ...
• Description
  Rabbit polyclonal to Tbx3
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Chicken
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Tbx3.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in nuclear extracts from the following cell lines: HepG2; MCF7.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Organogenesis
  • Angiogenesis and vasculogenesis

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • Other

Applications

Target

• FunctionTranscriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation.
• Tissue specificityWidely expressed.
• Involvement in diseaseDefects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands.
• Sequence similaritiesContains 1 T-box DNA-binding domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession O15119 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 374202 Chicken
  • Entrez Gene: 6926 Human
  • Entrez Gene: 21386 Mouse
  • Entrez Gene: 353305 Rat
  • Omim: 601621 Human
  • SwissProt: O15119 Human
  • SwissProt: P70324 Mouse
  • SwissProt: Q7TST9 Rat

  • Unigene: 129895 Human
  • Unigene: 219139 Mouse
  • Unigene: 162144 Rat

  see all

• Alternative names
  Bladder cancer related protein XHL antibodyT box 3 antibodyT-box protein 3 antibody

  T-box transcription factor TBX3 antibodyTbx3 antibodyTBX3 ISO antibodyTBX3_HUMAN antibodyUMS antibodyXHL antibody

  see all

Anti-Tbx3 antibody images

• 

  Western blot - Tbx3 antibody (ab99302)
  All lanes : Anti-Tbx3 antibody (ab99302) at 1 µg/ml
  
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
  Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate (ab14860)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 79 kDa
  Observed band size : 79 kDa
  Additional bands at : 22 kDa,77 kDa (possible isoform). We are unsure as to the identity of these extra bands.
  
  Exposure time : 3 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Tbx3 antibody (ab99302)

  ICC/IF image of ab99302 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99302 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HepG2 cells at 5ug/ml.