BMP4 Human ELISA Kit (ab99982)
- Product nameBMP4 Human ELISA KitSee all BMP4 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 15 pg/ml
- Range8.23 pg/ml - 6000 pg/ml
< 96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.25 85% - 104% Serum 92.95 80% - 101% Plasma 94.32 82% - 102%
- Species reactivityReacts with: Human
- Product overview
BMPs (Bone morphogenetic proteins) are found in minute amounts in bone material. BMP4 has been shown to be involved also in the differentiation of sympathetic neurons. It enhances the formation of adrenergic sympathetic neurons in cultures of neural crest cells.
ab99982 BMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml BMP-4 Microplate (12 strips x 8 wells) coated with anti-human BMP-4 1 unit BMP4 Standard Human (recombinant) 2 vials Detection Antibody BLMP-4: biotinylated anti-human BMP-4 2 vials HRP-Streptavidin Concentrate 80x concentrated HRP conjugated streptavidin Item G 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- BMP 2B BMP 4BMP2B
- BMP2B1BOMPR4ABone morphogenetic protein 2BBone morphogenetic protein 4DVR4OTTMUSP00000021065OTTMUSP00000021066ZYME
- SwissProt: P12644 Human
Our Abpromise guarantee covers the use of ab99982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for BMP4 Human ELISA Kit (ab99982)
ab99982 has not yet been referenced specifically in any publications.