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Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (AGIIVEPIQS EGGDNHASDD FFRKLRDIAR KHGCAFLVDE VQTGGGCTGK) of Human ABAT (NP_065737).
Our Abpromise guarantee covers the use of ab81432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/12500.
ab81432 staining ABAT in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab81432 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. ABAT I expression can be observed in amacrine cells (in green).
ab81432 has not yet been referenced specifically in any publications.