Overview

  • Product name
    Anti-ABAT antibody [EPR4433]
    See all ABAT primary antibodies
  • Description
    Rabbit monoclonal [EPR4433] to ABAT
  • Host species
    Rabbit
  • Specificity
    Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ABAT aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab202287)

  • Positive control
    • HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. antigen retrieval is recommended
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
  • Tissue specificity
    Liver > pancreas > brain > kidney > heart > placenta.
  • Involvement in disease
    Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
  • Sequence similarities
    Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • (S) 3 amino 2 methylpropionate transaminase antibody
    • (S)-3-amino-2-methylpropionate transaminase antibody
    • 4 aminobutyrate aminotransferase antibody
    • 4 aminobutyrate aminotransferase, mitochondrial antibody
    • 4-aminobutyrate aminotransferase antibody
    • ABAT antibody
    • FLJ17813 antibody
    • FLJ30272 antibody
    • GABA aminotransferase antibody
    • GABA AT antibody
    • GABA T antibody
    • GABA transaminase antibody
    • GABA transferase antibody
    • GABA-AT antibody
    • GABA-T antibody
    • GABAT antibody
    • GABT_HUMAN antibody
    • Gamma amino N butyrate transaminase antibody
    • Gamma-amino-N-butyrate transaminase antibody
    • hCG1984265 antibody
    • L AIBAT antibody
    • L-AIBAT antibody
    • LAIBAT antibody
    • mitochondrial antibody
    • NPD009 antibody
    see all

Images

  • All lanes : Anti-ABAT antibody [EPR4433] (ab108249)

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Human fetal liver tissue lysate
    Lane 4 : rat brain tissue lysate
    Lane 5 : rat kidney tissue lysate
    Lane 6 : mouse brain tissue lysate

    Predicted band size: 56 kDa

  • ab108249, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.
  • Overlay histogram showing HepG2 cells stained with ab108249 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108249, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Chen L  et al. Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatr Dis Treat 12:1731-7 (2016). WB . Read more (PubMed: 27471387) »
  • De Ita-Pérez D  et al. Synchronization by food access modifies the daily variations in expression and activity of liver GABA transaminase. Biomed Res Int 2014:590581 (2014). WB ; Rat . Read more (PubMed: 24809054) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Liver tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 14 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
breast cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 23 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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