Overview

  • Product nameAnti-ABCA1 antibody [AB.H10]
    See all ABCA1 primary antibodies
  • Description
    Mouse monoclonal [AB.H10] to ABCA1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
    Database link: O95477

  • Positive control
    • testis, liver, and brain tissue (negative control: muscle tissue)

Applications

Our Abpromise guarantee covers the use of ab18180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/200 - 1/500. Predicted molecular weight: 254 kDa.

Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.

ELISA Use at an assay dependent concentration.

Direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.

Target

  • FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • Tissue specificityWidely expressed, but most abundant in macrophages.
  • Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Post-translational
    modifications
    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 1 antibody
    • ABC Transporter 1 antibody
    • ABC-1 antibody
    • ABC1 antibody
    • ABCA 1 antibody
    • ABCA1 antibody
    • ABCA1_HUMAN antibody
    • ATP binding Cassette 1 antibody
    • ATP binding cassette sub family A ABC1 member 1 antibody
    • ATP binding cassette sub family A member 1 antibody
    • ATP binding Cassette Transporter 1 antibody
    • ATP-binding cassette 1 antibody
    • ATP-binding cassette sub-family A member 1 antibody
    • ATP-binding cassette transporter 1 antibody
    • CERP antibody
    • Cholesterol efflux regulatory protein antibody
    • FLJ14958 antibody
    • HDLDT1 antibody
    • Membrane bound antibody
    • MGC164864 antibody
    • MGC165011 antibody
    • TD antibody
    • TGD antibody
    see all

Anti-ABCA1 antibody [AB.H10] images



  • Predicted band size : 254 kDa
    Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
  • Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution

    Lane 1 : Wild type mouse liver tissue lysate
    Lane 2 : Knockout mouse liver tissue lysate
    Lane 3 : Wild type mouse liver tissue lysate
    Lane 4 : Knockout mouse liver tissue lysate
    Lane 5 : Wild type mouse liver tissue lysate
    Lane 6 : Knockout mouse liver tissue lysate

    Lysates/proteins at 100 µg per lane.


    Predicted band size : 254 kDa
    Observed band size : 254 kDa

    The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.

  • Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.

References for Anti-ABCA1 antibody [AB.H10] (ab18180)

This product has been referenced in:
  • Zhao X  et al. Perilipin1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice. PLoS One 10:e0123738 (2015). Mouse . Read more (PubMed: 25855981) »
  • Wagschal A  et al. Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nat Med 21:1290-7 (2015). Read more (PubMed: 26501192) »

See all 32 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing (7.5)
Loading amount 25 µg
Specification liver
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary astrocyte culture)
Permeabilization Yes - 0.1M PBS with 1% Triton X
Specification Primary astrocyte culture
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Methanol
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 06 2016

Application Western blot
Sample Rabbit Tissue lysate - whole (Synovial membrane)
Gel Running Conditions Reduced Denaturing (8 %)
Loading amount 35 µg
Specification Synovial membrane
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2016

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD PERMEABILISATION SOLUTION
Sample Mouse Cell (liver cells)
Specification liver cells
Gating Strategy CD24 POSITIVE
Preparation Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1%PBS-BSA
Username

Abcam user community

Verified customer

Submitted Dec 10 2013

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD Permeabilisation Solution
Sample Rat Cell (Liver cells)
Specification Liver cells
Gating Strategy CD24 Positive
Preparation Cell harvesting/tissue preparation method: Single cell suspension
Sample buffer: 0.1% PBS-BSA
Username

Abcam user community

Verified customer

Submitted Dec 05 2013

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD PERMEABILISATION SOLUTION
Sample Human Cell (HEP G2)
Specification HEP G2
Gating Strategy CD24 POSITIVE
Preparation Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1%PBS-BSA
Username

Mr. brahmi manali

Verified customer

Submitted Dec 04 2013

Application Western blot
Sample Pig Tissue lysate - whole (small intestine)
Loading amount 30 µg
Specification small intestine
Gel Running Conditions Reduced Denaturing (7)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted Apr 23 2013


Yes, I can send you as a one time exception a positive control lysate (rat liver (ab29413), http://www.abcam.com/liver-rat-whole-cell-lysate-normal-tissue-ab29413.html). This has been tested with ab66217 and gave strong bands.

Also I woul...

Read More

In this image the lower band is actin which has been recognized by and anti-actin antibody. This has been used as a loading control to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expressio...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 monocyte-derived macrophages)
Loading amount 50 µg
Specification THP-1 monocyte-derived macrophages
Gel Running Conditions Reduced Denaturing (8 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

Submitted Jan 04 2013

1-10 of 52 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"