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Recombinant fragment corresponding to ABCA1 (N terminal). 50 kDa N-terminal extracellular loop of ABCA1
Database link: O95477
Alternative versions available:
Our Abpromise guarantee covers the use of ab66217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 254 kDa (predicted molecular weight: 254 kDa).
Abcam recommends using 3% Milk as the blocking agent.
|Flow Cyt||Use 2µg for 106 cells. (methanol fixed cells)
ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|