Overview

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab14148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • ABC 1
    • ABC Transporter 1
    • ABC-1
    • ABC1
    • ABCA 1
    • ABCA1
    • ABCA1_HUMAN
    • ATP binding Cassette 1
    • ATP binding cassette sub family A ABC1 member 1
    • ATP binding cassette sub family A member 1
    • ATP binding Cassette Transporter 1
    • ATP-binding cassette 1
    • ATP-binding cassette sub-family A member 1
    • ATP-binding cassette transporter 1
    • CERP
    • Cholesterol efflux regulatory protein
    • FLJ14958
    • HDLDT1
    • Membrane bound
    • MGC164864
    • MGC165011
    • TD
    • TGD
    see all
  • FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • Tissue specificityWidely expressed, but most abundant in macrophages.
  • Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Post-translational
    modifications
    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • Cellular localizationMembrane.
  • Information by UniProt

References for ABCA1 peptide (ab14148)

This product has been referenced in:
  • Patel DC  et al. Type 2 Diabetes Is Associated with Reduced ATP-Binding Cassette Transporter A1 Gene Expression, Protein and Function. PLoS One 6:e22142 (2011). ELISA . Read more (PubMed: 21829447) »
  • Paul V  et al. A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J Lipid Res : (2008). ELISA ; Human . Read more (PubMed: 18541924) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. To clarify I can tell you that the KLH conjugation is actually a mistake on our datasheet. This peptide is NOT conjugated to a carrier molecule. I have amended the datasheet to reflect this. This should be evident tomorro...

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Thank you for your enquiry. I am sorry but following correspondence with the lab I can tell you that the KLH conjugation is actually a mistake. This antibody is NOT conjugated to a carrier molecule. I have amended the datasheet to reflect this. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"