Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

Overview

  • Product nameAnti-ABCA1 (phospho S2054) antibody [EPR2485]
    See all ABCA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)
  • Specificityab125064 only detects ABCA1 phosphorylated at serine 2054. Cross reactivity with mouse was determined by sequence analysis only.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCA1 aa 2050-2150 (phospho S2054). The exact sequence is proprietary.
    Database link: O95477

  • Positive control
    • WB: Okadaic Acid and Calyculin A treated SH-SY5Y cell lysates. ICC/IF: Okadaic Acid and Calyculin A treated SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab125064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 240 kDa (predicted molecular weight: 254 kDa).
ICC/IF 1/500 - 1/1000.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
    • Tissue specificityWidely expressed, but most abundant in macrophages.
    • Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
      Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
      Contains 2 ABC transporter domains.
    • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-2054 regulates phospholipid efflux.
      Palmitoylation by DHHC8 is essential for membrane localization.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC 1 antibody
      • ABC Transporter 1 antibody
      • ABC-1 antibody
      • ABC1 antibody
      • ABCA 1 antibody
      • ABCA1 antibody
      • ABCA1_HUMAN antibody
      • ATP binding Cassette 1 antibody
      • ATP binding cassette sub family A ABC1 member 1 antibody
      • ATP binding cassette sub family A member 1 antibody
      • ATP binding Cassette Transporter 1 antibody
      • ATP-binding cassette 1 antibody
      • ATP-binding cassette sub-family A member 1 antibody
      • ATP-binding cassette transporter 1 antibody
      • CERP antibody
      • Cholesterol efflux regulatory protein antibody
      • FLJ14958 antibody
      • HDLDT1 antibody
      • Membrane bound antibody
      • MGC164864 antibody
      • MGC165011 antibody
      • TD antibody
      • TGD antibody
      see all

    Anti-ABCA1 (phospho S2054) antibody [EPR2485] images

    • All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/200000 dilution

      Lane 1 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 (pS2054) peptide
      Lane 2 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 non-phospho peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 254 kDa
      Observed band size : 240 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% BSA/TBST.

    • All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/50000 dilution

      Lane 1 : SH-SY5Y cell lysate, untreated
      Lane 2 : SH-SY5Y cell lysate, Okadaic Acid and Calyculin A treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 254 kDa
    • ab125064, at a 1/500 dilution, staining ABCA1 (green) in Okadaic Acid and Calyculin A treated SH-SY5Y cells by immunofluorescence. DNA is marked in blue.

    References for Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

    ab125064 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"