• Product name
  • Description
    Rabbit polyclonal to ABCA12
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ABCA12 aa 1850-1950 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q86UK0

  • Positive control
    • This antibody gave a positive signal within WB in the following tissue lysates:Human Placenta; Mouse Testis; Human Brain; Human Fetal Brain. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab154037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 293 kDa (predicted molecular weight: 293 kDa).


  • Function
    Probable transporter involved in lipid homeostasis.
  • Tissue specificity
    Mainly expressed in the stomach, placenta, testis and fetal brain.
  • Involvement in disease
    Defects in ABCA12 are the cause of ichthyosis harlequin (HI) [MIM:242500]; also known as harlequin fetus. HI is a very severe skin disorder in which the neonate is born with a thick covering of armor-like scales. The skin dries out to form hard diamond-shaped plaques separated by fissures, resembling 'armor plating'. The normal facial features are severely affected, with distortion of the lips (eclabion), eyelids (ectropion), ears, and nostrils. Affected babies are often born prematurely and rarely survive the perinatal period.
    Defects in ABCA12 are the cause of ichthyosis lamellar type 2 (LI2) [MIM:601277]; also known as ichthyosis congenita IIB (ICR2B). LI is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. It is one the most severe forms of ichthyoses apparent at birth and persisting throughout life. LI patients are born encased in a tight, shiny, translucent covering called collodion membrane. Over the first weeks of life, the collodion membrane is gradually replaced by generalized large, dark brown, plate-like scales with minimal to no erythroderma. Tautness of facial skin commonly results in ectropion, eclabium and scarring alopecia of the scalp. Common complications are severe heat intolerance and recurrent ear infections.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Domain
    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC transporter A family member 12 antibody
    • ABC transporter ABCA.12 antibody
    • ABC12 antibody
    • ABCA12 antibody
    • ABCAC_HUMAN antibody
    • AtABCA12 antibody
    • ATH16 antibody
    • ATP binding cassette 12 antibody
    • ATP binding cassette sub family A (ABC1) member 12 antibody
    • ATP binding cassette sub family A member 12 antibody
    • ATP binding cassette transporter 12 antibody
    • ATP-binding cassette 12 antibody
    • ATP-binding cassette sub-family A member 12 antibody
    • ATP-binding cassette transporter 12 antibody
    • Ichthyosis congenita II lamellar ichthyosis B antibody
    • ICR2B antibody
    • LI2 antibody
    • Putative ABC2 homolog 16 antibody
    see all


  • All lanes : Anti-ABCA12 antibody (ab154037) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 293 kDa
    Observed band size: 293 kDa
    Additional bands at: 172 kDa (possible non-specific binding)

    Exposure time: 3 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154037 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of ABCA12 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154037, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab154037 has not yet been referenced specifically in any publications.

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