• Product nameAnti-ABCA4 antibody [3F4]
    See all ABCA4 primary antibodies
  • Description
    Mouse monoclonal [3F4] to ABCA4
  • Tested applicationsSuitable for: IHC-FoFr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Xenopus laevis
  • Immunogen

    Partially purified bovine 220 kDa disc rim protein.

  • Positive control
    • Adult mouse retina tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab77285 is protein G purified from culture supernatant.
  • ClonalityMonoclonal
  • Clone number3F4
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab77285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 20436469
WB 1/1000. Predicted molecular weight: 257 kDa.
IHC-P 1/100.


  • FunctionIn the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.
  • Tissue specificityRetinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells.
  • Involvement in diseaseDefects in ABCA4 are the cause of Stargardt disease type 1 (STGD1) [MIM:248200]. STGD is one of the most frequent causes of macular degeneration in childhood. It is characterized by macular dystrophy with juvenile-onset, rapidly progressive course, alterations of the peripheral retina, and subretinal deposition of lipofuscin-like material. STGD1 inheritance is autosomal recessive.
    Defects in ABCA4 are the cause of fundus flavimaculatus (FFM) [MIM:248200]. FFM is an autosomal recessive retinal disorder very similar to Stargardt disease. In contrast to Stargardt disease, FFM is characterized by later onset and slowly progressive course.
    Defects in ABCA4 may be a cause of age-related macular degeneration type 2 (ARMD2) [MIM:153800]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in ABCA4 are the cause of cone-rod dystrophy type 3 (CORD3) [MIM:604116]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
    Defects in ABCA4 are the cause of retinitis pigmentosa type 19 (RP19) [MIM:601718]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP19 is characterized by choroidal atrophy. Inheritance is autosomal recessive.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane. Localized to outer segment disk edges of rods and cones, with around one million copies/photoreceptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 10 antibody
    • ABC A4 antibody
    • ABC transporter, retinal-specific antibody
    • ABC10 antibody
    • ABCA 4 antibody
    • abcA4 antibody
    • ABCA4_HUMAN antibody
    • ABCR antibody
    • ARMD 2 antibody
    • ARMD2 antibody
    • ATP binding cassette 10 antibody
    • ATP binding cassette sub family A member 4 antibody
    • ATP binding cassette sub family A member4 antibody
    • ATP binding cassette transporter antibody
    • ATP binding cassette transporter retinal specific antibody
    • ATP binding cassette, sub family A (ABC1), member 4 antibody
    • ATP binding cassette, sub family A (ABC1), member4 antibody
    • ATP binding cassette10 antibody
    • ATP binding transporter, retina specific antibody
    • ATP-binding cassette sub-family A member 4 antibody
    • CORD 3 antibody
    • CORD3 antibody
    • DKFZp781N1972 antibody
    • FFM antibody
    • FLJ17534 antibody
    • Photoreceptor rim protein antibody
    • Retina specific ABC transporter antibody
    • Retinal specific ATP binding cassette transporter antibody
    • Retinal-specific ATP-binding cassette transporter antibody
    • RIM ABC transporter antibody
    • RIM protein antibody
    • RmP antibody
    • RP 19 antibody
    • RP19 antibody
    • Stargardt disease protein antibody
    • STGD antibody
    • STGD1 antibody
    see all

Anti-ABCA4 antibody [3F4] images

  • ab77285, at a 1/100 dilution, staining ABCA4 in formalin fixed, paraffin embedded adult mouse retina tissue by Immunohistochemisty.
  • All lanes : Anti-ABCA4 antibody [3F4] (ab77285) at 1/500 dilution

    Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Rat Retina Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 257 kDa
    Observed band size : 257 kDa
    Additional bands at : 142 kDa,51 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-ABCA4 antibody [3F4] (ab77285)

This product has been referenced in:
  • Partida GJ  et al. Thy1 associates with the cation channel subunit HCN4 in adult rat retina. Invest Ophthalmol Vis Sci 53:1696-703 (2012). WB ; Rat . Read more (PubMed: 22281825) »
  • Beaty TH  et al. A genome-wide association study of cleft lip with and without cleft palate identifies risk variants near MAFB and ABCA4. Nat Genet 42:525-9 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20436469) »

See all 2 Publications for this product

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