Anti-ABCB11 antibody (ab112494)

Overview

  • Product nameAnti-ABCB11 antibody
    See all ABCB11 primary antibodies
  • Description
    Rabbit polyclonal to ABCB11
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to a sequence at the C-terminal of Human ABCB11.

Properties

Applications

Our Abpromise guarantee covers the use of ab112494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 146 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionInvolved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
  • Tissue specificityExpressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
  • Involvement in diseaseDefects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
    Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC member 16, MDR/TAP subfamily antibody
    • ABCB1 antibody
    • Abcb11 antibody
    • ABCBB_HUMAN antibody
    • ATP binding cassette sub family B (MDR/TAP) member 11 antibody
    • ATP binding cassette sub family B member 11 antibody
    • ATP-binding cassette sub-family B member 11 antibody
    • Bile salt export pump antibody
    • BRIC2 antibody
    • Bsep antibody
    • PGY4 antibody
    • progressive familial intrahepatic cholestasis 2 antibody
    • Sister of P glycoprotein antibody
    • Spgp antibody
    see all

Anti-ABCB11 antibody images

  • All lanes : Anti-ABCB11 antibody (ab112494)

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Mouse liver tissue lysate
    Lane 5 : SMMC whole cell lysate


    Predicted band size : 146 kDa
    Observed band size : 146 kDa

References for Anti-ABCB11 antibody (ab112494)

ab112494 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: w/ or w/o 0.01M Sodium Cytrate pH 6.0
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative 4% PFA + 30% Sucrose
Username

Abcam user community

Verified customer

Submitted Oct 07 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Acetone or 0.1% Acetic Acid/Ethanol
Username

Abcam user community

Verified customer

Submitted Oct 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Sodium Cytrate pH 6.0
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 07 2014

Thank you for your inquiry.


We have 5 antibodies to ABCB11, but unfortunately they were all raised to cytoplasmic regions.


We continually add antibodies and other protein detection products to our catalogue but we do not...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDCK)
Loading amount 50 µg
Specification MDCK
Treatment transfected with human-BSEP
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 12 2012

Thank you for contacting us.

Regarding the western blot data for ab112494, the laboratory routinely uses 30˜40μg of liver sample.

The secondary antibody dilution will vary depending on which secondary antibody is used and which de...

Read More

Thank you for contacting us.

I have contacted the lab regarding ab112494, and will let you know what I find out.

As for ab78279, since the antibody is not in the catalog anymore, I am actuallynotable to send a testing discount code...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"