Anti-ABCB11 antibody - Carboxyterminal end (ab71793)

Overview

  • Product name
    Anti-ABCB11 antibody - Carboxyterminal end
    See all ABCB11 primary antibodies
  • Description
    Rabbit polyclonal to ABCB11 - Carboxyterminal end
  • Specificity
    We have received some negative reports from customers using this antibody in IHC, therefore we do not recommend this antibody for IHC.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C-terminal region of human ABCB11.

  • Positive control
    • Human hepatocarcinoma tissue and mouse liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa).
ELISA 1/1000.

Target

  • Function
    Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
  • Tissue specificity
    Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
  • Involvement in disease
    Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
    Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Domain
    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC member 16, MDR/TAP subfamily antibody
    • ABC16 antibody
    • ABCB1 antibody
    • Abcb11 antibody
    • ABCBB_HUMAN antibody
    • ATP binding cassette sub family B (MDR/TAP) member 11 antibody
    • ATP binding cassette sub family B member 11 antibody
    • ATP-binding cassette sub-family B member 11 antibody
    • Bile salt export pump antibody
    • BRIC2 antibody
    • Bsep antibody
    • PFIC 2 antibody
    • PFIC2 antibody
    • PGY4 antibody
    • progressive familial intrahepatic cholestasis 2 antibody
    • Sister of P glycoprotein antibody
    • Spgp antibody
    see all

Images

  • Anti-ABCB11 antibody - Carboxyterminal end (ab71793) at 1/100 dilution + mouse liver tissue lysate at 12.5 µg

    Predicted band size : 146 kDa
    Observed band size : 146 kDa

References

ab71793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products and that you do not observe a staining in IHC. We take product complaints very seriously, and investigate every product that we feel may not be p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human hepatoma cells Huh7)
Loading amount
25 µg
Specification
Human hepatoma cells Huh7
Treatment
0.5uM GW4064
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2011

Thank you for your enquiry. I have contacted the originator of the product for more information, unfortunately the exact immunogen sequence information is proprietary. However, they did released information that the sequence is a 15 amino acid prot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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