Anti-ABCB5 antibody (ab77549)
Key features and details
- Goat polyclonal to ABCB5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ABCB5 antibody
See all ABCB5 primary antibodies -
Description
Goat polyclonal to ABCB5 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Chimpanzee -
Immunogen
Synthetic peptide:
C-QTQHRNTSKKAQ
, corresponding to internal sequence amino acids 460-471 of Human ABCB5 (NP_848654.3). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. -
Tissue specificity
Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 2 ABC transporter domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 340273 Human
- Omim: 611785 Human
- SwissProt: Q2M3G0 Human
- Unigene: 404102 Human
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Alternative names
- ABCB 5 antibody
- ABCB5 antibody
- ABCB5 P-gp antibody
see all
Images
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Anti-ABCB5 antibody (ab77549) at 0.03 µg/ml + A431 cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 90 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. -
Anti-ABCB5 antibody (ab77549) at 0.3 µg/ml + Human skin Lysate in RIPA buffer. at 35 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (6)
ab77549 has been referenced in 6 publications.
- Louphrasitthiphol P et al. ABCB5 is activated by MITF and ß-catenin and is associated with melanoma differentiation. Pigment Cell Melanoma Res 33:112-118 (2020). PubMed: 31595650
- Boulding T et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). PubMed: 29311580
- Keniya MV et al. Drug resistance is conferred on the model yeast Saccharomyces cerevisiae by expression of full-length melanoma-associated human ATP-binding cassette transporter ABCB5. Mol Pharm 11:3452-62 (2014). PubMed: 25115303
- Lai CY et al. CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry. Cancer Res 72:5111-8 (2012). IHC-P ; Human . PubMed: 22865455
- Kupas V et al. RANK is expressed in metastatic melanoma and highly upregulated on melanoma-initiating cells. J Invest Dermatol 131:944-55 (2011). Flow Cyt ; Human . PubMed: 21270824
- Cheung ST et al. Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance. Gastroenterology 140:344-55 (2011). Flow Cyt ; Human . PubMed: 20682318