Overview

  • Product nameAnti-ABCB5 antibody
    See all ABCB5 primary antibodies
  • Description
    Goat polyclonal to ABCB5
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-QTQHRNTSKKAQ

    , corresponding to internal sequence amino acids 460-471 of Human ABCB5 (NP_848654.3).

  • Positive control
    • A431 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 20682318ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ELISA Use at an assay dependent concentration. antibody detection limit dilution 1:32000.
IHC-P Use at an assay dependent concentration. PubMed: 22865455

Target

  • FunctionPlasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas.
  • Tissue specificityExpressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 2 ABC transporter domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCB 5 antibody
    • ABCB5 antibody
    • ABCB5 P-gp antibody
    • ABCB5_HUMAN antibody
    • ABCB5alpha antibody
    • ABCB5beta antibody
    • ATP binding cassette protein antibody
    • ATP binding cassette sub family B (MDR/TAP) member 5 antibody
    • ATP binding cassette sub family B member 5 antibody
    • ATP-binding cassette sub-family B member 5 antibody
    • EST422562 antibody
    • P glycoprotein ABCB5 antibody
    • P-glycoprotein ABCB5 antibody
    see all

Anti-ABCB5 antibody images

  • Anti-ABCB5 antibody (ab77549) at 0.03 µg/ml + A431 cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 90 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Anti-ABCB5 antibody (ab77549) at 0.3 µg/ml + Human skin Lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa

References for Anti-ABCB5 antibody (ab77549)

This product has been referenced in:
  • Lai CY  et al. CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry. Cancer Res 72:5111-8 (2012). IHC-P ; Human . Read more (PubMed: 22865455) »
  • Cheung ST  et al. Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance. Gastroenterology 140:344-55 (2011). Flow Cyt ; Human . Read more (PubMed: 20682318) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that the immunogen for this antibody is located in the internal sequence amino acids 460-471 of Human ABCB5. The amino acids are intracellular according to SwissProt:

http://www.uniprot.org/...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"