Overview

  • Product nameAnti-ABCB9 antibody
    See all ABCB9 primary antibodies
  • Description
    Rabbit polyclonal to ABCB9
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 475 and 525 of human ABCB9 (NP_062571.1)

  • Positive control
    • HeLa, 293T and mouse NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab86222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceThe membrane associated protein encoded by this gene is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications.
  • Cellular localizationIntegral membrane protein. Lysosomal. May be localized in the endoplasmic reticulum.
  • Database links
  • Alternative names
    • ABC transporter 9 protein antibody
    • ABCB 9 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 9 antibody
    • ATP binding cassette sub family B member 9 precursor antibody
    • ATP binding cassette transporter 9 antibody
    • EST122234 antibody
    • hABCB9 antibody
    • KIAA1520 antibody
    • TAP like protein antibody
    • TAPL antibody
    see all

Anti-ABCB9 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ABCB9 with ab86222 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ABCB9 antibody (ab86222) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 84 kDa
    Observed band size : 84 kDa


    Exposure time : 10 seconds
  • Detection of human ABCB9 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86222 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

References for Anti-ABCB9 antibody (ab86222)

This product has been referenced in:
  • Rodriguez-Navarro JA  et al. Inhibitory effect of dietary lipids on chaperone-mediated autophagy. Proc Natl Acad Sci U S A 109:E705-14 (2012). WB ; Mouse . Read more (PubMed: 22331875) »

See 1 Publication for this product

Product Wall

Unfortunately, no we do not have the blocking peptide commercially available for this product. I apologize for the inconvenience.

Thank you for confirmation.

The antibody ab45371 is currently out of stock until 14th January. The items will be dispatched on 15th January with order number 1233746.

I hope this information will be helpful. Should you have any questi...

Read More

Thank very much for your patience while laboratory was retesting the specificity of this antibody.

I can confirm that the antibody has passed the quality test again in reciprocal IP/WB so we have no doubt about the specificity of this product....

Read More

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

Just to update you regarding further steps. Our laboratory has agreed for further testing of this antibody. The results are expected by t...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"