The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 174 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.
Involvement in disease
Defects in ABCC9 are the cause of cardiomyopathy dilated type 1O (CMD1O) [MIM:608569]; also known as dilated cardiomyopathy with ventricular tachycardia. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in ABCC9 are the cause of familial atrial fibrillation type 12 (ATFB12) [MIM:614050]. ATFB12 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
ATP-binding cassette sub-family C member 9 antibody
ATP-binding cassette transporter sub-family C member 9 antibody
ATP-binding cassette, sub-family C (CFTR/MRP), member 9 antibody
Sulfonylurea receptor 2 antibody
Sulfonylurea-binding protein 2 antibody
Western blot - Anti-ABCC9 antibody (ab84299)
Western blot analysis of 10µg SUR1 KO (lane 1) 10µg WT (lane 2) 0.1µg SUR1 overexpressing (lane 3) mouse ventricle lysates and 10µg cannine ventricle lysate (lane 4) labeling ABCC9 with ab84299 at 1:1000. A HRP-conjugated anti-rabbit (1:2000) was used as the secondary antibody.
Western blot - ABCC9 antibody (ab84299)
Anti-ABCC9 antibody (ab84299) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 174 kDa Observed band size: 174 kDa Additional bands at: 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.