The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAntagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCE family. Contains 2 4Fe-4S ferredoxin-type domains. Contains 2 ABC transporter domains.
Cellular localizationCytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.