Overview

  • Product nameAnti-ABCF1 antibody
    See all ABCF1 primary antibodies
  • Description
    Rabbit polyclonal to ABCF1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 150 and 200 of human ABCF1 (NP_001020262.1)

  • Positive control
    • HeLa and 293T whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 96 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • FunctionIsoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
    Contains 2 ABC transporter domains.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2; inhibits association of EIF2 with ribosomes.
  • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC27 antibody
    • ABC50 antibody
    • ABCF1 antibody
    • ABCF1_HUMAN antibody
    • ATP binding cassette 50 (TNF alpha stimulated) antibody
    • ATP binding cassette 50 antibody
    • ATP binding cassette sub family F member 1 antibody
    • ATP binding cassette, sub family F (GCN20), member 1 antibody
    • ATP-binding cassette 50 antibody
    • ATP-binding cassette sub-family F member 1 antibody
    • AU041969 antibody
    • D17Wsu166e antibody
    • EST123147 antibody
    • GCN20 antibody
    • TNF alpha stimulated ABC protein antibody
    • TNF-alpha-stimulated ABC protein antibody
    • TNFalpha inducible ATP binding protein antibody
    • wu:fb79c06 antibody
    • wu:fc39a05 antibody
    • wu:fj94a08 antibody
    • zgc:85667 antibody
    see all

Anti-ABCF1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling ABCF1 with ab86223 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ABCF1 antibody (ab86223) at 0.025 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 96 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of human ABCF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86223 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 1 second.

References for Anti-ABCF1 antibody (ab86223)

ab86223 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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