Overview

  • Product nameAnti-ABCF2 antibody
    See all ABCF2 primary antibodies
  • Description
    Rabbit polyclonal to ABCF2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human ABCF2 using the numbering given in entry NP_009120.1.

  • Positive control
    • HeLa whole cell lysate 293T whole cell lysate NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 71 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-ABCF2 antibody images

    • All lanes : Anti-ABCF2 antibody (ab86178) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Observed band size : 71 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds
    • Detection of Human ABCF2 by Immunoprecipitation, using ab86178 at 3µg/mg lysate. Image shows immunoprecipitated ABCF2 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with ab86178 at 1 µg/ml in lane 1, antibodies recognising a downstream eptiope in lanes 2 and 3, and control IgG in lane 4. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-ABCF2 antibody (ab86178)

    ab86178 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86178.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"