Overview

  • Product nameAnti-ABCG1 antibodySee all ABCG1 primary antibodies ...
  • Description
    Rabbit polyclonal to ABCG1
  • Tested applicationsIHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids 300-400 of Human ABCG1 (Peptide available as ab97573.)

  • Positive control
    • Mouse peritoneal macrophages.

Applications

Our Abpromise guarantee covers the use of ab36969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.
WB 1/2500. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).

Target

  • FunctionTransporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
  • Tissue specificityExpressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
  • Target information above from: UniProt accession P45844 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ABC transporter 8 antibody
    • Abc8 antibody
    • ABCG1 antibody
    • ABCG1_HUMAN antibody
    • ATP-binding cassette sub family G member 1 antibody
    • ATP-binding cassette sub-family G member 1 antibody
    • ATP-binding cassette transporter 8 antibody
    • ATP-binding cassette transporter member 1 of subfamily G antibody
    • ATP-binding cassette, sub family G WHITE member 1 antibody
    • homolog of Drosophila white antibody
    • MGC34313 antibody
    • White protein homolog antibody
    • White protein homolog ATP binding cassette transporter 8 antibody
    • WHITE1 antibody
    • wht1 antibody
    see all

Anti-ABCG1 antibody images

  • All lanes : Anti-ABCG1 antibody (ab36969)

    Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
    Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
    Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
    Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)

    developed using the ECL technique

    Predicted band size : 76 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa (possible non-specific binding).
  • ab36969 staining ABCG1 in lung and alveolar macrophages by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-ABCG1 antibody (ab36969)

This product has been referenced in:
  • Voloshyna I  et al. Plasma from rheumatoid arthritis patients promotes pro-atherogenic cholesterol transport gene expression in THP-1 human macrophages. Exp Biol Med (Maywood) 238:1192-7 (2013). WB ; Human . Read more (PubMed: 24000379) »
  • Wang Y  et al. Supplementation with cyanidin-3-O-ß-glucoside protects against hypercholesterolemia-mediated endothelial dysfunction and attenuates atherosclerosis in apolipoprotein E-deficient mice. J Nutr 142:1033-7 (2012). Mouse . Read more (PubMed: 22535762) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages)
Loading amount 30 µg
Specification macrophages
Treatment acLDL 24h
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 02 2012

Application Western blot
Sample Human Cell lysate - whole cell (THP1, HEK293)
Loading amount 15 µg
Specification THP1, HEK293
Treatment 1uM TO-1317
Gel Running Conditions Reduced Denaturing
Blocking step 5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

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Verified customer

Submitted Jan 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"