The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionTransporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
Tissue specificityExpressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.
References for Anti-ABCG1 antibody [EP1366Y] (ab52617)
This product has been referenced in:
Hu YW et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res55:681-97 (2014).
Read more (PubMed: 24493833) »
Adlakha YK et al. Pro-apoptotic miRNA-128-2 modulates ABCA1, ABCG1 and RXRa expression and cholesterol homeostasis. Cell Death Dis4:e780 (2013).
Read more (PubMed: 23990020) »