Overview

  • Product nameAnti-ABCG1 antibody [EP1366Y]
    See all ABCG1 primary antibodies
  • Description
    Rabbit monoclonal [EP1366Y] to ABCG1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    A synthetic peptide corresponding to residues on human ABCG1.

  • Positive control
    • HUVEC cell lysate, human cervical carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Applications

Our Abpromise guarantee covers the use of ab52617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionTransporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
    • Tissue specificityExpressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC transporter 8 antibody
      • ABC8 antibody
      • ABCG1 antibody
      • ABCG1_HUMAN antibody
      • ATP-binding cassette sub family G member 1 antibody
      • ATP-binding cassette sub-family G member 1 antibody
      • ATP-binding cassette transporter 8 antibody
      • ATP-binding cassette transporter member 1 of subfamily G antibody
      • ATP-binding cassette, sub family G WHITE member 1 antibody
      • homolog of Drosophila white antibody
      • MGC34313 antibody
      • White protein homolog antibody
      • White protein homolog ATP binding cassette transporter 8 antibody
      • WHITE1 antibody
      • WHT1 antibody
      see all

    Anti-ABCG1 antibody [EP1366Y] images

    • All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat liver lysate
      Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
      Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
      Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

      Predicted band size : 75 kDa

      Blocking and diluting buffer: 5% NFDM /TBST

    • ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

    • Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antiibody at 1/2000 dilution

      Predicted band size : 75 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.

    References for Anti-ABCG1 antibody [EP1366Y] (ab52617)

    This product has been referenced in:
    • Lin YW  et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. J Mol Cell Cardiol 79:287-94 (2015). WB ; Mouse . Read more (PubMed: 25528964) »
    • Hu YW  et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014). Read more (PubMed: 24493833) »

    See all 13 Publications for this product

    Product Wall

    Application Western blot
    Sample Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 35 µg
    Specification Synovial membrane
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain)
    Antigen retrieval step None
    Permeabilization Yes - 0.1M PBST with 10%donkeys serum
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 12 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions Reduced Denaturing
    Loading amount 30 µg
    Specification Brain lysate, Liver lysate and human serum
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jan 28 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HepG2)
    Specification HepG2
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 13 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Cow Cell lysate - whole cell (endothelial cells from cerebral capillaries)
    Loading amount 20 µg
    Specification endothelial cells from cerebral capillaries
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Dr. GOSSELET Fabien

    Verified customer

    Submitted Mar 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"