A synthetic peptide corresponding to residues on human ABCG1.
HUVEC cell lysate, human cervical carcinoma.
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
Is unsuitable for IHC-P.
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
Western blot - Anti-ABCG1 antibody [EP1366Y] (ab52617)
Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg
Secondary Goat anti-rabbit HRP antiibody at 1/2000 dilution
References for Anti-ABCG1 antibody [EP1366Y] (ab52617)
This product has been referenced in:
Na TY et al. The trisaccharide raffinose modulates epidermal differentiation through activation of liver X receptor. Sci Rep7:43823 (2017).
Read more (PubMed: 28266648) »
Cheng-Mao X et al. Placental ABCA1 Expression Is Increased in Spontaneous Preterm Deliveries Compared with Iatrogenic Preterm Deliveries and Term Deliveries. Biomed Res Int2017:8248094 (2017).
WB, IHC, RT-PCR
Read more (PubMed: 28630870) »