Recombinant Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1366Y] to ABCG1 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
Related conjugates and formulations
Overview
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Product name
Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free
See all ABCG1 primary antibodies -
Description
Rabbit monoclonal [EP1366Y] to ABCG1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HUVEC cell lysate.
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General notes
ab218528 is the carrier-free version of ab52617.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1366Y -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. -
Tissue specificity
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes. - Information by UniProt
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Database links
- Entrez Gene: 510745 Cow
- Entrez Gene: 9619 Human
- Entrez Gene: 11307 Mouse
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- SwissProt: Q64343 Mouse
- Unigene: 124649 Human
see all -
Alternative names
- ABC transporter 8 antibody
- ABC8 antibody
- ABCG1 antibody
see all
Images
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ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52617).
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Lane 1 : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/1000 dilution
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
All lanes : C-Myc/DDK-tagged full length human ABCG1 recombinant protein 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 180 secondsThis data was developed using the same antibody clone in a different buffer formulation (ab52617).
Blocking/Dilution buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (15)
ab218528 has been referenced in 15 publications.
- Yin H et al. Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells. Oncogene 39:3754-3773 (2020). PubMed: 32157214
- Li HN et al. miR-146a-5p suppresses ATP-binding cassette subfamily G member 1 dysregulation in patients with refractory Mycoplasma pneumoniae via interleukin 1 receptor-associated kinase 1 downregulation. Int J Mol Med 44:2003-2014 (2019). PubMed: 31638178
- Lin YW et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. J Mol Cell Cardiol 79:287-94 (2015). WB ; Mouse . PubMed: 25528964
- Hu YW et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014). PubMed: 24493833
- Adlakha YK et al. Pro-apoptotic miRNA-128-2 modulates ABCA1, ABCG1 and RXRa expression and cholesterol homeostasis. Cell Death Dis 4:e780 (2013). WB ; Human . PubMed: 23990020