Overview

  • Product nameAnti-ABCG5 antibody
    See all ABCG5 primary antibodies
  • Description
    Rabbit polyclonal to ABCG5
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human ABCG5.

    (Peptide available as ab87830.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; SW480; Ramos.

Properties

Applications

Our Abpromise guarantee covers the use of ab87116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionTransporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
  • Tissue specificityStrongly expressed in the liver, lower levels in the small intestine and colon.
  • Involvement in diseaseDefects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abcg5 antibody
    • ABCG5_HUMAN antibody
    • ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody
    • ATP binding cassette sub family G member 5 antibody
    • ATP-binding cassette sub-family G member 5 antibody
    • ATP-binding cassette, sub-family G (WHITE), member 5 antibody
    • Sterolin 1 antibody
    • Sterolin-1 antibody
    • STSL antibody
    see all

Anti-ABCG5 antibody images

  • All lanes : Anti-ABCG5 antibody (ab87116) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,68 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab87116 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87116, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ABCG5 antibody (ab87116)

ab87116 has not yet been referenced specifically in any publications.

Product Wall

I now have more information to share with you in regards to the immunogen used to raise the anti-ABCG5 antibody ab124965. As can be seen from the attachment, the immunogen used to raise this antibody also does not share high homology with the rat prote...

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Thank you for contacting us and your interest in our products.
The ABCG5 antibodies which we have are all directed against the human protein. This shares 79% homology with the rat protein. We have not tested any of the anti-ABCG5 antibodies we hav...

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