Recombinant
RabMAb

Anti-ABCG5 antibody [EPR6203] (ab124965)

Overview

  • Product name
    Anti-ABCG5 antibody [EPR6203]
    See all ABCG5 primary antibodies
  • Description
    Rabbit monoclonal [EPR6203] to ABCG5
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ABCG5.

  • Positive control
    • Ramos and HepG2 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
    • Tissue specificity
      Strongly expressed in the liver, lower levels in the small intestine and colon.
    • Involvement in disease
      Defects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abcg5 antibody
      • ABCG5_HUMAN antibody
      • ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody
      • ATP binding cassette sub family G member 5 antibody
      • ATP-binding cassette sub-family G member 5 antibody
      • ATP-binding cassette, sub-family G (WHITE), member 5 antibody
      • Sterolin 1 antibody
      • Sterolin-1 antibody
      • STSL antibody
      see all

    Images

    • All lanes : Anti-ABCG5 antibody [EPR6203] (ab124965) at 1/1000 dilution

      Lane 1 : Ramos cell lysates
      Lane 2 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 72 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124965 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    I now have more information to share with you in regards to the immunogen used to raise the anti-ABCG5 antibody ab124965. As can be seen from the attachment, the immunogen used to raise this antibody also does not share high homology with the rat prote...

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    Thank you for contacting us and your interest in our products.
    The ABCG5 antibodies which we have are all directed against the human protein. This shares 79% homology with the rat protein. We have not tested any of the anti-ABCG5 antibodies we hav...

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