Overview

  • Product name
  • Description
    Rabbit polyclonal to ABCG8
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding corresponding to amino acids 299-415 of Human ABCG8.

  • Positive control
    • Human duodenum tissue; RT 4 and U251 MG cell lysate, Human Plasma lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab126493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500. Predicted molecular weight: 76 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
  • Tissue specificity
    Strongly expressed in the liver, lower levels in the small intestine and colon. Detectable in a wide variety of human tissues.
  • Involvement in disease
    Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4) [MIM:611465]. With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries.
    Defects in ABCG8 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCG8 antibody
    • ABCG8_HUMAN antibody
    • ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody
    • ATP binding cassette sub family G member 8 antibody
    • ATP binding cassette, sub family G (WHITE), member 8 antibody
    • ATP-binding cassette sub-family G member 8 antibody
    • GBD4 antibody
    • MGC142217 antibody
    • Sterolin 2 antibody
    • Sterolin-2 antibody
    • STSL antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab126493 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126493 at 1/50 dilution staining ABCG8 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-ABCG8 antibody (ab126493) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

    Predicted band size : 76 kDa

References

This product has been referenced in:
  • Castella B  et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). Read more (PubMed: 28580927) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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