Overview

  • Product name
  • Description
    Rabbit polyclonal to ABH2
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (VPRKQATYGD AGLTYTFSGL TLSPKPWIPV LERIRDHVSG VTGQTFNFVL) of Human ABH2 (NP_001001655).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificity
    Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • Sequence similarities
    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Nucleus. Detected in replication foci during S-phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2OG Fe(II) oxy DC1 antibody
    • 9530023G02 antibody
    • ABH 2 antibody
    • ABH2 antibody
    • alkB alkylation repair homolog 2 antibody
    • ALKB2_HUMAN antibody
    • ALKBH2 alkB antibody
    • ALKBH2 antibody
    • Alkylated DNA repair protein alkB homolog antibody
    • Alkylated DNA repair protein alkB homolog 2 antibody
    • Alkylation repair homolog 2 antibody
    • Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody
    • AU016977 antibody
    • DNA oxidative demethylase ALKBH2 antibody
    • EC 1.14.11. antibody
    • FLJ99103 antibody
    • hABH2 antibody
    • mABH2 antibody
    • MGC90512 antibody
    • Oxy DC1 antibody
    see all

Images

  • Anti-ABH2 antibody (ab83005) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.

References

ab83005 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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