Recombinant
RabMAb

Anti-ABH2 antibody [EPR6177(2)] (ab154859)

Overview

  • Product name
    Anti-ABH2 antibody [EPR6177(2)]
    See all ABH2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6177(2)] to ABH2
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ABH2 (Uniprot ID Q6NS38).

  • Positive control
    • Human fetal brain, Human testis, Jurkat and HeLa lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab154859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
    • Tissue specificity
      Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Nucleus. Detected in replication foci during S-phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2OG Fe(II) oxy DC1 antibody
      • 9530023G02 antibody
      • ABH 2 antibody
      • ABH2 antibody
      • alkB alkylation repair homolog 2 antibody
      • ALKB2_HUMAN antibody
      • ALKBH2 alkB antibody
      • ALKBH2 antibody
      • Alkylated DNA repair protein alkB homolog antibody
      • Alkylated DNA repair protein alkB homolog 2 antibody
      • Alkylation repair homolog 2 antibody
      • Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody
      • AU016977 antibody
      • DNA oxidative demethylase ALKBH2 antibody
      • EC 1.14.11. antibody
      • FLJ99103 antibody
      • hABH2 antibody
      • mABH2 antibody
      • MGC90512 antibody
      • Oxy DC1 antibody
      see all

    Images

    • All lanes : Anti-ABH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Human testis tissue lysate
      Lane 3 : Human fetal brain tissue lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
    • Flow cytometric analysis of permeabilized Jurkat cells labeling ABH2 with ab154859 at 1/100 (red). Rabbit IgG control (green).

    References

    ab154859 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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