Anti-ABHD12 antibody (ab68949)
Key features and details
- Mouse polyclonal to ABHD12
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ABHD12 antibody -
Description
Mouse polyclonal to ABHD12 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ABHD12. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- ABHD12-transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Has 2-arachidonoylglycerol hydrolase activity (By similarity). May be a regulator of endocannabinoid signaling pathways. -
Involvement in disease
Defects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC) [MIM:612674]. PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses. -
Sequence similarities
Belongs to the serine esterase family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 26090 Human
- Omim: 613599 Human
- SwissProt: Q8N2K0 Human
- Unigene: 441550 Human
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Alternative names
- 2-arachidonoylglycerol hydrolase antibody
- 6330583M11Rik antibody
- ABD12_HUMAN antibody
see all
Images
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All lanes : Anti-ABHD12 antibody (ab68949) at 1/500 dilution
Lane 1 : ABHD12 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Datasheets and documents
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Datasheet download
References (2)
ab68949 has been referenced in 2 publications.
- Joshi A et al. Biochemical characterization of the PHARC-associated serine hydrolase ABHD12 reveals its preference for very-long-chain lipids. J Biol Chem 293:16953-16963 (2018). PubMed: 30237167
- Navia-Paldanius D et al. Biochemical and pharmacological characterization of human a/ß-hydrolase domain containing 6 (ABHD6) and 12 (ABHD12). J Lipid Res 53:2413-24 (2012). WB . PubMed: 22969151