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Rabbit polyclonal to ABHD2
ab74680 detects endogenous levels of total ABHD2 protein.
Predicted to work with:
Synthetic peptide derived from an internal sequence of human ABHD2.
HT29 cell extracts and MCF7 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
1/500 - 1/1000.
ABHD2 contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function ABHD2 remains unknown but it may play a role in smooth muscle cell migration. Alternative splicing of this gene results in two transcript variants encoding the same protein.
Membrane; Single-pass type II membrane protein
abhydrolase domain containing 2 antibody
Abhydrolase domain-containing protein 2 antibody
Immunocytochemistry/ Immunofluorescence - ABHD2 antibody (ab74680)
ab74680 at 1/500-1/1000 dilution staining ABHD2 in MCF7 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
Western blot - ABHD2 antibody (ab74680)
All lanes : Anti-ABHD2 antibody (ab74680) at 1/500 dilution Lane 1 : HT29 cell extracts Lane 2 : HT29 cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 49 kDa Observed band size: 49 kDa Additional bands at: 100 kDa, 26 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"