Overview

  • Product name
  • Description
    Rabbit polyclonal to ABHD2
  • Specificity
    ab74680 detects endogenous levels of total ABHD2 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human ABHD2.

  • Positive control
    • HT29 cell extracts and MCF7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab74680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    ABHD2 contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function ABHD2 remains unknown but it may play a role in smooth muscle cell migration. Alternative splicing of this gene results in two transcript variants encoding the same protein.
  • Cellular localization
    Membrane; Single-pass type II membrane protein
  • Database links
  • Alternative names
    • 2210009N18Rik antibody
    • abhydrolase domain containing 2 antibody
    • Abhydrolase domain-containing protein 2 antibody
    • alpha/beta hydrolase domain containing protein 2 antibody
    • HS1 2 antibody
    • Labh 2 antibody
    • LABH2 antibody
    • Lung alpha/beta hydrolase 2 antibody
    • MGC107122 antibody
    • MGC111112 antibody
    • MGC26249 antibody
    • PHPS1 2 antibody
    • protein PHPS1-2 antibody
    see all

Images

  • ab74680 at 1/500-1/1000 dilution staining ABHD2 in MCF7 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-ABHD2 antibody (ab74680) at 1/500 dilution

    Lane 1 : HT29 cell extracts
    Lane 2 : HT29 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 100 kDa,26 kDa,95 kDa. We are unsure as to the identity of these extra bands.

References

ab74680 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74680.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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