The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceABHD2 contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function ABHD2 remains unknown but it may play a role in smooth muscle cell migration. Alternative splicing of this gene results in two transcript variants encoding the same protein.
Cellular localizationMembrane; Single-pass type II membrane protein