• Product nameAnti-Abhd5 antibody
    See all Abhd5 primary antibodies
  • Description
    Rabbit polyclonal to Abhd5
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: IPVSVIFGAR SCIDGNSGTS IQSLRPHSYV KTIAILGAGH YVYADQPEEF, corresponding to C terminal amino acids 288-337 of Human Abhd5 (NP_057090).

  • Positive control
    • MCF7 cell lysate.


Our Abpromise guarantee covers the use of ab80365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionLysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • Tissue specificityWidely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • Involvement in diseaseDefects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • Sequence similaritiesBelongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • Developmental stageDetected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localizationCytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
    • ABHD5 antibody
    • ABHD5_HUMAN antibody
    • Abhydrolase domain containing 5 antibody
    • Abhydrolase domain containing protein 5 antibody
    • Abhydrolase domain-containing protein 5 antibody
    • CDS antibody
    • CGI 58 antibody
    • CGI58 antibody
    • CGI58 protein antibody
    • IECN2 antibody
    • Lipid droplet-binding protein CGI-58 antibody
    • MGC8731 antibody
    • NCIE2 antibody
    see all

Anti-Abhd5 antibody images

  • Anti-Abhd5 antibody (ab80365) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab80365 staining Abhd5 in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80365, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry of Human Colon lysate tissue at an antibody concentration of 10µg/ml using anti-ABHD5 antibody (ab80365)

References for Anti-Abhd5 antibody (ab80365)

This product has been referenced in:
  • Orban T  et al. Retinyl ester storage particles (retinosomes) from the retinal pigmented epithelium resemble lipid droplets in other tissues. J Biol Chem 286:17248-58 (2011). WB ; Cow . Read more (PubMed: 21454509) »

See 1 Publication for this product

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