Recombinant
RabMAb

Anti-Abhd5 antibody [EPR12621] (ab183739)

Overview

  • Product name
    Anti-Abhd5 antibody [EPR12621]
    See all Abhd5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12621] to Abhd5
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Abhd5 aa 150-250. The exact sequence is proprietary.
    Database link: Q8WTS1

  • Positive control
    • HepG2 cells and cell lysates; U87-MG and Human fetal liver cell lysates; Human lipomyoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
Flow Cyt 1/200. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • Tissue specificity
    Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • Involvement in disease
    Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • Sequence similarities
    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • Developmental stage
    Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
    • ABHD5 antibody
    • ABHD5_HUMAN antibody
    • Abhydrolase domain containing 5 antibody
    • Abhydrolase domain containing protein 5 antibody
    • Abhydrolase domain-containing protein 5 antibody
    • CDS antibody
    • CGI 58 antibody
    • CGI58 antibody
    • CGI58 protein antibody
    • IECN2 antibody
    • Lipid droplet-binding protein CGI-58 antibody
    • MGC8731 antibody
    • NCIE2 antibody
    see all

Images

  • All lanes : Anti-Abhd5 antibody [EPR12621] (ab183739) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 39 kDa
  • Anti-Abhd5 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 39 kDa
  • Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

References

This product has been referenced in:
  • Rittig N  et al. Regulation of Lipolysis and Adipose Tissue Signaling during Acute Endotoxin-Induced Inflammation: A Human Randomized Crossover Trial. PLoS One 11:e0162167 (2016). Read more (PubMed: 27627109) »
  • Christensen B  et al. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis 15:160 (2016). WB ; Human . Read more (PubMed: 27640183) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - whole (adipose tissue)
Specification
adipose tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Ms. Helle Zibrandtsen

Verified customer

Submitted Mar 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up